Schellekens_BLongum_Obesity_Stress_EBIOM_103176 (003)


Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human
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Keywords
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Abstract
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Background
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Methods
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Findings
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Interpretation
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Funding
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Ev­i­dence be­fore this study
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Added value of this study
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Im­pli­ca­tions of avail­able ev­i­dence
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Introduction
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Methods – animal study
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Animals, diets and ethical approval
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In vivo probiotic administration
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In vivo glucose tolerance test
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Murine tissue sampling
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Murine biochemical analysis
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Murine RNA isolation and quantitative real-time PCR
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Methods – human intervention study
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Human intervention study outline
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Inclusion and exclusion criteria
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Study setting and ethical approval
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Randomisation and blinding
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Study recruitment
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Product formulation and dosage
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Collection and analysis of blood samples
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Collection and analysis of cortisol awakening response samples
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Methods – murine and human microbiota
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Murine and human microbiota sequencing
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Faecal SCFA quantification
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Questionnaires
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Statistical analysis
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Results
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B. longum APC1472 decreases body weight gain and fat depots accumulation in obese mice
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B. longum APC1472 administration improves glucose tolerance, circulating levels of leptin and corticosterone in obese mice
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B. longum APC1472 induces changes of hypothalamic neuropeptide expression in mice
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Human intervention study population
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Adverse events
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B. longum APC1472 does not affect BMI and W/H ratio in humans
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B. longum APC1472 improves fasting glucose levels independent of other blood markers of energy metabolism and satiety in humans
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B. longum APC1472 does not influence human lipid and inflammatory profiles in humans
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B. longum APC1472 does not affect satiety, mood, perceived stress and cortisol awakening response in humans
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B. longum APC1472 improves fasting glucose levels, active ghrelin and cortisol awakening response in obese individuals
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B. longum APC1472 does not induce major rearrangements on the microbiota composition but increases the abundance of Bifidobacterium
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Discussion
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Data sharing
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Disclosure
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Contributions
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Uncited references
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Acknowledgements
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Supplementary materials
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Footnotes
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Article Footnotes
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References
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atlantiaclinicaltrials.com

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