Pathogens 2021 , 10 , 235
5of 18
Table1. Effects on microbial community composition as assessed through qPCR. The average increase in Lactobacillus and Bifidobacterium levels (16S rRNA gene copies/mL for lumen and 16S rRNA gene copies/g for mucus) during the treatment period as compared to the control period upon treatment with OFO at a dose of 1.4 g/d and POF at a dose of 0.6 g/d, 1.0 g/d, and 1.4 g/d in the luminal and mucosal environment of the proximal (PC) and distal colon (DC) of the human gastrointestinal tract for three human donors tested. For optimal observation of consistent effects over the different donors tested, the average of the three donors is presented ( n = 3). The intensity of the shading correlates with the absolute abundance, which is normalised for each of the different bacterial groups in the different colonic environments, respectively. Statistically significant differences relative to the control period are indicated with underlining ( p < 0.05). Statistically significant differences between the different test conditions within a colonic region are indicated with different letters (a, b, c), with conditions not sharing any similar letter being significantly different from each other ( p <0.05).
PC
DC
POF 0.6g/d
POF 1.0g/d
POF 1.4g/d
OFO 1.4g/d
POF 0.6g/d
POF 1.0g/d
POF 1.4g/d
OFO 1.4g/d
a a
a
a
a a
3.5 × 10 8 2.4 × 10 9
1.4 × 10 9 4.0 × 10 9 9.0 × 10 9 1.4 × 10 10
6.2 × 10 9 3.3 × 10 9 4.5 × 10 9 9.2 × 10 9
4.3 × 10 8 1.2 × 10 9 7.2 × 10 9 1.0 × 10 10
1.5 × 10 8 6.0 × 10 7 1.4 × 10 9 2.3 × 10 8
9.1 × 10 8 b,c 7.8 × 10 7 a,b
5.6 × 10 9 1.3 × 10 9 4.3 × 10 9 1.1 × 10 9
2.8 × 10 8 a,c
b b b b
b b
Lumen Mucus Lumen Mucus
Lactobacillus
a
8.7 × 10 7 3.6 × 10 9 5.6 × 10 8
a,b
a,b
a a
a a
a
4.5 × 10 9 a,b 5.3 × 10 9 a,b
4.2 × 10 9 5.8 × 10 8
b
b
b
Bifidobacterium
Powered by FlippingBook